exosome in cancer
Sunday 5 January 2014
Exosomes are small membrane vesicles released by most cell types including tumor cells. The intercellular exchange of proteins and genetic material via exosomes is a potentially effective approach for cell-to-cell communication and it may perform multiple functions aiding to tumor survival and metastasis.
Exosome in cancer
- The exosome has been shown to be inhibited by the antimetabolite drug fluorouracil, which is a drug used in chemotherapy treatment of cancer. It is one of the most successful drugs for treating solid tumors.
- In yeast cells treated with fluorouracil, defects were seen in the processing of ribosomal RNA identical to those seen when the activity of the exosome was blocked by molecular biological strategies.
- Lack of correct ribosomal RNA processing is lethal to cells, explaining the antimetabolic effect of the drug.
- MicroRNA and protein profiling of brain metastasis competent cell-derived exosomes. doi : 10.1371/journal.pone.0073790 PMID: 24066071
It has been developed working procedures to evaluate exosomal RNA signature as novel biomarkers for cancer prognosis, prediction and patient stratification.
In particular, it has refreshed current practice and demonstrated a new approach for studying RNA signature in patient blood samples.
MicroRNA and protein profiling of brain metastasis competent cell-derived exosomes. Camacho L, Guerrero P, Marchetti D. PLoS One. 2013 Sep 16;8(9):e73790. doi : 10.1371/journal.pone.0073790 PMID: 24066071 (Free)
Proteomic profiling of exosomes leads to the identification of novel biomarkers for prostate cancer. Duijvesz D, Burnum-Johnson KE, Gritsenko MA, Hoogland AM, Vredenbregt-van den Berg MS, Willemsen R, Luider T, Paša-Tolić L, Jenster G. PLoS One. 2013 Dec 31;8(12):e82589. doi : 10.1371/journal.pone.0082589 PMID: 24391718 [Free]
The roles of extracellular vesicles in cancer biology: towards the development of novel cancer biomarkers. Katsuda T, Kosaka N, Ochiya T. Proteomics. 2013 Dec 12. doi : 10.1002/pmic.201300389 PMID: 24339442
Prostate Cancer Biomarker Profiles in Urinary Sediments and Exosomes. Dijkstra S, Birker IL, Smit FP, Leyten GH, de Reijke TM, van Oort IM, Mulders PF, Jannink SA, Schalken JA. J Urol. 2013 Nov 7. pii: S0022-5347(13)05884-9. doi : 10.1016/j.juro.2013.11.001 PMID: 24211598
Clinical use of novel urine and blood based prostate cancer biomarkers: A review. Dijkstra S, Mulders PF, Schalken JA. Clin Biochem. 2013 Oct 29. pii: S0009-9120(13)00497-9. doi : 10.1016/j.clinbiochem.2013.10.023 PMID: 24177197