IHC techniques

• translocation-associated IHC
  2 May 2017

  translocation-associated immunochemistry
  Examples
  translocation-associated immunohistochemical stains in the fine-needle aspiration diagnosis of salivary gland neoplasms. 2016. doi:10.1002/cncy.21693. PLAG1 HMGA2 MYB KIT

• PD-L1 study
  31 October 2016

  PD-L1 IHC; PD-L1 immunochemistry
  Indications
  VENTANA PD-L1 assay (SP142 assay) before atezolizumab (TECENTRIQ®) treatment previously treated metastatic non-small cell lung carcinoma (NSCLC ) urothelial carcinoma
  non-small cell lung cancer (NSCLC) Predictive PD-L1 immunohistochemistry for non-small cell lung cancer
  Antibodies
  Ventana SP142
  Ventana SP263
  Cell signaling E1L3N
  Dako 22C3 PharmDx
  Dako 28-8 PharmDx (...)

• immunochemistry reactivity
  8 August 2016

  Types
  nuclear reactivity
  cytoplasmic reactivity cytoplasmic granular reactivity cytoplasmic dot-like reactivity
  nuclear and cytoplasmic reactivity
  membranous reactivity
  See also
  immunochemistry

• Immunohistochemical markers
  25 February 2015

  3 beta  7 AAD  11A1  14-3-3sigma  15-PGDH  45M1
  A A beta 42  abl  acid phosphatase  ACSM1   actin-general  actin-alpha cardiac  actin-alpha smooth muscle  actin-muscle specific  AE1/AE3 (cytokeratin)  AF4  AGR2  AKT1  albumin  alcian blue  ALK  alkaline phosphatase  ALL1  alpha-1-antichymotrypsin  alpha-1-antitrypsin  alpha-fetoprotein  alpha-lactalbumin  AMACR  AML1  androgen receptor  APC  API2-MALT1  Apolipoprotein D  arginase1  ATM 
  B72.3  - bcl1 - bcl2 bcl6 bclXl bcr-abl Beclin1 BerEP4  (...)

• cytoplasmic positivity of TTF-1
  22 May 2013

  cytoplasmic positivity of TTF1 eIHC
  Cytoplasmic staining with TTF-1 has been little studied. This cytoplasmic staining is due to a cross-reacting antigen of 160 kd, which is not an alternative splicing product of TTF-115.
  In a large series of consecutive cases, about 6% (23/361) of all tumours from a variety of sites showed cytoplasmic staining.
  hepatocellular carcinoma
  a minority of pulmonary adenocarcinomas show coarse granular cytoplasmic staining
  occasionally in squamous (...)

• CA-125
  22 May 2013

  WP PO
  CA-125 (cancer antigen 125 or carbohydrate antigen 125) also known as "mucin 16" or "MUC16" is a protein that in humans is encoded by the MUC16 gene.
  MUC16 is a member of the mucin family glycoproteins.
  CA-125 has found application as a tumor marker or biomarker that may be elevated in the blood of some patients with specific types of cancers, or other benign conditions.

• Anti-Cytokeratin (CAM 5.2)
  12 February 2013

  Anti-Cytokeratin (CAM 5.2) reagent has a primary reactivity with human keratin proteins that correspond to Moll’s peptides #7 and #8, Mr 48 and 52 kilodaltons (kd), respectively.
  Cytokeratin 7 and 8 are present on secretory epithelia of normal human tissue but not on stratified squamous epithelium.
  Anti-Cytokeratin (CAM 5.2) stains most epithelial-derived tissue, including liver, renal tubular epithelium, and hepatocellular and renal cell carcinomas.
  Anti-Cytokeratin (CAM 5.2) might not (...)

• cytokeratin 5/6
  26 September 2012

  Pathology
  All squamous carcinomas of the lung express cytokeratin 5/6.
  Cytokeratin 5/6 reactivity was observed in all 40 mesotheliomas, but there was none in any of the 30 pulmonary adenocarcinomas.
  The cytokeratin 5/6 immunostaining is useful in separating epithelial pleural mesotheliomas from pulmonary adenocarcinomas but also can assist in distinguishing epithelial mesotheliomas from nonpulmonary adenocarcinomas metastatic to the pleura.
  Reference
  Value of cytokeratin 5/6 (...)

• ProExC
  10 August 2012

  ProExC expression has been shown to perform similarly to p16 as an aid in the diagnosis of cervical dysplasia but has not been well characterized in head and neck squamous cell carcinomas (SCC).
  ProExC does not perform well enough to be used as a prognostic marker in oropharyngeal SCC.
  References
  Evaluation of ProExC as a Prognostic Marker in Oropharyngeal Squamous Cell Carcinomas. Mills AM, Beck AH, Pourmand N, Le QT, Kong CS. Am J Surg Pathol. 2012 Aug;36(8):1158-64. PMID: (...)

• ERG immunochemistry
  2 August 2012

  References
  The diagnostic utility of novel immunohistochemical marker ERG in the workup of prostate biopsies with "atypical glands suspicious for cancer". He H, Magi-Galluzzi C, Li J, Carver P, Falzarano S, Smith K, Rubin MA, Zhou M. Am J Surg Pathol. 2011 Apr;35(4):608-14. PMID: #21383613#
  ERG transcription factor as an immunohistochemical marker for vascular endothelial tumors and prostatic carcinoma. Miettinen M, Wang ZF, Paetau A, Tan SH, Dobi A, Srivastava S, Sesterhenn I. Am J (...)

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