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Home > Technical section > Biology > Chromosomal study > EM-ISEL


Wednesday 18 July 2007

EM-ISEL is used to assess DNA single-strand breaks (SSBs) expressed as number of immunogold particles per microm2 of chromatin at both chromosomal and nuclear DNA levels.

See also

- genotoxicity
- DNA single-strand breaks (SSBs)


- Depault F, Cojocaru M, Fortin F, Chakrabarti S, Lemieux N. Genotoxic effects of chromium(VI) and cadmium(II) in human blood lymphocytes using the electron microscopy in situ end-labeling (EM-ISEL) assay. Toxicol In Vitro. 2006 Jun;20(4):513-8. PMID: 16278061

- Assad M, Yahia LH, Rivard CH, Lemieux N. In vitro biocompatibility assessment of a nickel-titanium alloy using electron microscopy in situ end-labeling (EM-ISEL). J Biomed Mater Res. 1998 Jul;41(1):154-61. PMID: 9641635

- Assad M, Lemieux N, Rivard CH. Immunogold electron microscopy in situ end-labeling (EM-ISEL): assay for biomaterial DNA damage detection. Biomed Mater Eng. 1997;7(6):391-400. PMID: 9622107

- Migheli A, Attanasio A, Schiffer D. Ultrastructural detection of DNA strand breaks in apoptotic neural cells by in situ end-labelling techniques. J Pathol. 1995 May;176(1):27-35. PMID: 7542332