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BRAF-V600E IHC

Wednesday 25 February 2015

IHC for BRAF V600E is relatively sensitive and specific, but there is a discordance rate that is not trivial with NGS mutation profiling. In addition, a significant proportion of patients harbor BRAF non-V600E not detectable by IHC, potentially limiting utility of IHC screening for BRAF mutations. (25634750)

References

- NRASQ61R and BRAFV600E Mutation-specific Immunohistochemistry Is a Helpful Tool to Diagnose Metastatic Undifferentiated/Dedifferentiated Melanomas: Agaimy A, Mentzel T. Am J Surg Pathol. 2016 Mar 31. PMID: 27035613

- Comparison of Next-generation Sequencing Mutation Profiling With BRAF and IDH1 Mutation-specific Immunohistochemistry. Jabbar KJ, Luthra R, Patel KP, Singh RR, Goswami R, Aldape KD, Medeiros LJ, Routbort MJ. Am J Surg Pathol. 2015 Jan 28. PMID: 25634750