Immunochemistry

The effective implementation of personalized cancer therapeutic regimens depends on the successful identification and translation of informative biomarkers to aid clinical decision making.
Antibody-based proteomics occupies a pivotal space in the cancer biomarker discovery and validation pipeline, facilitating the high-throughput evaluation of candidate markers.
Although the clinical utility of these emerging technologies remains to be established, the traditional use of antibodies as (...)

Links
Dako HercepTest webpage
Breast
This HercepTest™ Interpretation Manual - Breast is provided as a tool to help guide pathologists and laboratorians to achieve correct and reproducible results.
The goal of this manual is to familiarize you with the requirements for scoring breast carcinomas stained with HercepTest™.
Example cases of various staining intensities of HER2 expression are provided for reference.
The HercepTest™ package insert guidelines will be reviewed and technical tips (...)

HER2 immunochemistry; HER2 IHC, ERBB IHC; HER2 quantification
The human epidermal growth factor receptor 2 (HER2) is an established biomarker for management of patients with breast cancer.
HER2 quantification
The currently FDA-approved methods for HER2 quantification are not able to give a quantitative measure of HER2 heterogeneity of expression. It has been developed an immunofluorescence assay to quantify HER2 on FFPE samples. Coupled with automated image analysis, this assay is able (...)

HPA

Examples
VEGFR3
Prospero-related homeobox gene-1 [Prox-1 or PROX1]
D2-40

References
Shi C, Zhou G, Zhu Y, Su Y, Cheng T, Zhau HE, Chung LW. Quantum dots-based multiplexed immunohistochemistry of protein expression in human prostate cancer cells. Eur J Histochem. 2008 Apr-Jun;52(2):127-34. PMID: #18591159#
Maysinger D, Lovrić J. Quantum dots and other fluorescent nanoparticles: quo vadis in the cell? Adv Exp Med Biol. 2007;620:156-67. PMID: #18217342#
Gao X, Dave SR. Quantum dots for cancer molecular imaging. Adv Exp Med Biol. 2007;620:57-73. PMID: (...)

Purified monoclonal antibodies production
HuCal AbD serotec

Videos
See also
microscopy

tissue profiling