The effective implementation of personalized cancer therapeutic regimens depends on the successful identification and translation of informative biomarkers to aid clinical decision making.
Antibody-based proteomics occupies a pivotal space in the cancer biomarker discovery and validation pipeline, facilitating the high-throughput evaluation of candidate markers.
Although the clinical utility of these emerging technologies remains to be established, the traditional use of antibodies as (...)
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Immunochemistry
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antibody-based proteomics
9 March 2013, by admin -
HercepTest™ Interpretation Manual
7 December 2011Links
Dako HercepTest webpage
Breast
This HercepTest™ Interpretation Manual - Breast is provided as a tool to help guide pathologists and laboratorians to achieve correct and reproducible results.
The goal of this manual is to familiarize you with the requirements for scoring breast carcinomas stained with HercepTest™.
Example cases of various staining intensities of HER2 expression are provided for reference.
The HercepTest™ package insert guidelines will be reviewed and technical tips (...) -
ERBB2 immunochemistry
28 November 2011HER2 immunochemistry; HER2 IHC, ERBB IHC; HER2 quantification
The human epidermal growth factor receptor 2 (HER2) is an established biomarker for management of patients with breast cancer.
HER2 quantification
The currently FDA-approved methods for HER2 quantification are not able to give a quantitative measure of HER2 heterogeneity of expression. It has been developed an immunofluorescence assay to quantify HER2 on FFPE samples. Coupled with automated image analysis, this assay is able (...) -
The Human Protein Atlas
25 November 2011HPA
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lymphatic endothelium-specific antibodies
8 January 2010Examples
VEGFR3
Prospero-related homeobox gene-1 [Prox-1 or PROX1]
D2-40 -
quantum dots-based multiplexed immunohistochemistry
10 July 2008References
Shi C, Zhou G, Zhu Y, Su Y, Cheng T, Zhau HE, Chung LW. Quantum dots-based multiplexed immunohistochemistry of protein expression in human prostate cancer cells. Eur J Histochem. 2008 Apr-Jun;52(2):127-34. PMID: #18591159#
Maysinger D, Lovrić J. Quantum dots and other fluorescent nanoparticles: quo vadis in the cell? Adv Exp Med Biol. 2007;620:156-67. PMID: #18217342#
Gao X, Dave SR. Quantum dots for cancer molecular imaging. Adv Exp Med Biol. 2007;620:57-73. PMID: (...) -
monoclonal antibodies
10 July 2008Purified monoclonal antibodies production
HuCal AbD serotec -
immunofluorescence
21 June 2005Videos
See also
microscopy -
tissue microarrays
2 March 2004tissue profiling