microscopic imaging techniques
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Microscopy
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microscopy
21 January 2008 -
cryo-electron tomography
23 November 2006Cryo-electron tomography is an emerging imaging technique that has unique potential for molecular cell biology. At the present resolution of 4-5 nm, large supramolecular structures can be studied in unperturbed cellular environments.
In the future, it will become possible to map molecular landscapes inside cells in a more comprehensive manner.
’Visual proteomics’ aims to complement and extend mass-spectrometry-based inventories, and to provide a quantitative description of the (...) -
fluorescence microscopy
24 May 2005References
Pepperkok R, Ellenberg J. High-throughput fluorescence microscopy for systems biology. Nat Rev Mol Cell Biol. 2006 Sep;7(9):690-6. PMID: #16850035#
Egner A, Hell SW. Fluorescence microscopy with super-resolved optical sections. Trends Cell Biol. 2005 Apr;15(4):207-15. PMID: #15817377# -
diagnostic algorithms
1 May 2004diagnosis algorithms
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frozen section
18 March 2004frozen sections
Guidelines
CCR/NCI: http://home.ccr.cancer.gov/lop/intranet/ResidentsManual/TechniquesoftheFrozenSection.pdf
See also
intra-operative diagnostics
References
Kawamoto T. Use of a new adhesive film for the preparation of multi-purpose fresh-frozen sections from hard tissues, whole-animals, insects and plants. Arch Histol Cytol. 2003 May;66(2):123-43. PMID: (...) -
electron microscopy
2 December 2003References
Griffiths G. Bringing electron microscopy back into focus for cell biology. Trends Cell Biol. 2001 Apr;11(4):153-4. PMID: #11354032# -
evanescent wave microscopy
2 December 2003References
Toomre D, Manstein DJ. Lighting up the cell surface with evanescent wave microscopy. Trends Cell Biol. 2001 Jul;11(7):298-303. PMID: #11413041# -
quantitative multidimensional microscopy
2 December 2003References
Kam Z, Zamir E, Geiger B. Probing molecular processes in live cells by quantitative multidimensional microscopy. Trends Cell Biol. 2001 Aug;11(8):329-34. PMID: #11489638#